Order number: S5371.0296Shipping: shipped and stored at RT
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
96-well Plate PCR DNA Purification kit enabling purification of PCR products of 50bp – 30kb. PCR DNA is separated from DyeTerminators, Nucleotides, Primer or Primer dimers with high yield (>90%) and purity within 40 minutes. As already known from our spin column purification kits, even highly concentrated DNA can be isolated with high yields and high purity. DNA fragments purified with the HTS purification kit are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
The principle: The DNA purification procedure utilizes 96-minicolumns plates with membranes which efficiently and selectively bind nucleic acids. In the first step the CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane. As an added convenience, the binding buffer contains a colour indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. The purified DNA is eluted using either a low ionic strength buffer (Elution Buffer) or water (pH7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.
S5303 > (MicroPrep DNA Purification Kit) - S5364 > (Plasmid Midi DNA Purification Kit) - S5368 > (PCR DNA Purification Mini Prep Kit) - S5369 > (Plasmid DNA Purification Mini Prep Kit) - S5375 > (Blood DNA Purification Mini Prep Kit) S5378 > (Tissue Genomic DNA Purification Mini Prep Kit) - S5396 > (Genomic Bacteria DNA Purification Kit) or S5398 > (Universal Genomic DNA Purification Mini Spin Kit) and others >.
Yield: 80% to 95% (depending on fragment length)
Purity of isolated DNA: A260/A280 ratio: 1.7 – 1.9
Binding capacity: 20µg DNA per well
Time required: 40 min. per plate
Volume: up to 100µL per well
application:High-throughput removal of nucleases, enzyme inhibitors, detergents, enzymes, proteins, divalent ions, salts etc.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on 96-well Plate PCR DNA Purification Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.