Order number: S5383.0296Shipping: shipped and stored at RT
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
96-well Plate Plasmid DNA Purification Kit for the rapid and efficient purification of high quality plasmid DNA from recombinant Escherichia coli strains. The isolation protocol and buffer formulations were optimised for high isolation efficiency and DNA purity. The DNA purification procedure utilises special membrane technology which efficiently and selectively binds plasmid DNA that can be used irectly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.
The principle: In the first step, the plasmid DNA is released from bacterial cells by alkaline lysis. Then the lysate is neutralized and all the cell residues along with the proteins and genomic DNA are removed in a centrifugation step. The clear lysate is applied to the purification column membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified plasmid DNA is eluted using a low ionic strength buffer (Elution Buffer) or water (pH7.0-9.0) and can be used for further applications.
S5303 > (MicroPrep DNA Purification Kit) - S5364 > (Plasmid Midi DNA Purification Kit) - S5368 > (PCR DNA Purification Mini Prep Kit) - S5369 > (Plasmid DNA Purification Mini Prep Kit) - S5375 > (Blood DNA Purification Mini Prep Kit) S5378 > (Tissue Genomic DNA Purification Mini Prep Kit) - S5396 > (Genomic Bacteria DNA Purification Kit) or S5398 > (Universal Genomic DNA Purification Mini Spin Kit) and others >.
Purity of isolated DNA: A260/A280 = 1.7 - 1.9. Binding capacity: approx. 25µg DNA per well. Yield: depending on fragment length. From 0.5 - 10mL culture broth. Time per run: ca. 25 min. per plate.
application:PCR, qPCR, Southern blotting, DNA sequencing, NGS, cloning, enzymatic restriction, etc.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on 96-well Plate Plasmid DNA Purification Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.