96-well Plate Plasmid DNA Purification Kit

96-well Plate Plasmid DNA Purification Kit for the rapid and efficient purification of high quality plasmid DNA from recombinant Escherichia coli strains. The isolation protocol and buffer formulations were optimised for high isolation efficiency and  DNA purity. The DNA purification procedure utilises special membrane technology which efficiently and selectively binds plasmid DNA that can be used irectly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

The principle: In the first step, the plasmid DNA is released from bacterial cells by alkaline lysis. Then the lysate is neutralized and all the cell residues along with the proteins and genomic DNA are removed in a centrifugation step. The clear lysate is applied to the purification column membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified plasmid DNA is eluted using a low ionic strength buffer (Elution Buffer) or water (pH7.0-9.0) and can be used for further applications.

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