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Linear acrylamide (5 mg/mL) serves as a nucleic acid coprecipitation reagent and is ideal for purification of RNA and DNA samples used for PCR and real-time PCR reactions, as small amounts of contaminating nucleic acids can be amplified if other carriers are used, for example tRNA from yeast.
Linear acrylamide has been shown to precipitate picogram amounts of DNA fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides. This feature makes linear acrylamide useful for separating reaction products from unincorporated nucleotides and oligonucleotide primers.
Like glycogen, it is preferred over yeast RNA as a coprecipitant for applications where added nucleic acid could interfere or compete with subsequent enzymatic reactions. Linear acrylamide offers the additional advantage that it is not derived from a biological source or treated with reagents (e.g., proteases) derived from biological sources. For this it might be the most appropriate coprecipitant to use when precipitating DNA and RNA for PCR and RT-PCR reactions, respectively. In these procedures, small amounts of contaminating nucleic acids present in the carrier could also be amplified, generating spurious background.
1. Gaillard C and Strauss F (1990) Ethanol precipitation of DNA with linear polyacrylamide as a carrier. Nucleic Acids Research 18:378.
2. Wang E (2005) RNA amplification for successful gene profiling analysis. J Transl Med. 25(3):28
synthetic co-precipitant free of nucleic acid impurities.
5mg/mL solution in nuclease free water
for precipitation of picogram amounts of DNA longer than 20 bp
application:- co-precipitation / DNA-precipitation of picogram amounts of DNA longer than 20bp - no precipitation of DNA of fragments smaller 20bp or of nucleotides
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
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