CentriPure Mini Dye Terminator columns

CentriPure CP-0219 columns


Order number: CP-0219-Z025

Shipping: Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

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Die CentriPure CP-0219 columns are specially designed for purification and desalting of... more
Product information "CentriPure Mini Dye Terminator columns"

Die CentriPure CP-0219 columns are specially designed for purification and desalting of oligonucleotides longer than 20 base pairs and from proteins greater than 25 kDa with minimal dilution.

The gel bed has a volume of 0.5mL. Samples with a volume up to 100µL can be processed. Optimal purification and recovery is obtained with a sample volume of 50µL.

Because the columns are rehydrated shortly before use, their shelf life (non-hydrated) at +15°C to +30°C is several years.

More than 99% of impurities as salts, dNTPs or Dye terminators can be removed by our CentriPure columns. As a result you will get consitent and reliable signals from base 1 to 700.

Applications:

- Purification of DNA sequencing reactions.
- Removal of free and labelled dNTP’s from DNA/RNA polymerisation reactions (e.g. RT-PCR, PCR, nick translation).
- Purification/desalting of proteins. Removal of traces of phenol or exchange of buffer salts, as in multiple restriction digestions. These columns are far superior - in ease of use, speed, and non-toxicity - to such common techniques as phenol/chloroform extractions and ethanol precipitations.

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Specifications: Gel Matrix:  Zetadex-50SF Gel bed volume: 0.5mL Buffer conductifity: Less... more
 

Technical Data:

Specifications:
Gel Matrix:  Zetadex-50SF
Gel bed volume: 0.5mL
Buffer conductifity: Less than 300µS/cm
Sample volume: 20µL to 100µL
Optimal sample size 20µL to 50µL
DNase activity: Not detected; 

application:

- Purification of DNA sequencing reactions. - Removal of free and labelled dNTP’s from DNA/RNA polymerisation reactions (e.g. RT-PCR, PCR, nick translation). - Purification/desalting of proteins. Removal of traces of phenol or exchange of buffer salts, as

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-17-03-02
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Here you will find information and further literature on CentriPure Mini Dye Terminator columns. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.