Order number: S5350.0005Shipping: shipped at RT, store at +2°C to +8°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
WorkBeads Protein A is a ready-to-use resin. No additional mixing or reagents are required. It is not necessary to warm the product to room temperature before use. Protein A is a surface protein originally found in the cell wall of bacteria Staphylococcus aureus, Group G Streptococci and Peptostreptococcus magnus, respectively. Protein A and protein G bind with high affinity and specificity to Fc region of immunoglobulins from many of mammalian species, most notably IgGs. Unlike protein A and G, Protein L binds immunoglobulins through kappa light chain. These proteins have been usually used to purify, immobilize or detect immunoglobulins.
Advantages compared to competitor products:
- Higher dynamic binding capacity for monoclonal antibodies;
- Excellent recovery & high purity
- Stronger coupling chemistry; high pH stability and low leakage
- Reliable, reproducible and efficient
The product is supplied as a 50% suspension in 20% Ethanol. 10mL of the shipped product for example are equivalent to 5mL pure Resin plus 5mL solvent.
Low ligand leakage. Compatible with all standard aqueous buffers used for protein purification. pH stability: pH3–10 short term; pH2–12 for cleaning.
Target substance: Polyclonal and monoclonal antibodies, bound via the Fc-region.
Matrix: Rigid, highly cross-linked agarose.
Average particle size (Dv50): 45µm.
50% suspension in 20% ethanol.
Ligand: Recombinant protein A expressed in E. coli using animal-free medium.
Dynamic binding capacity (DBC) of typically more than 40mg IgG per 1mL medium.
Recommended flow rate: 250 cm/h (aqueous buffers).
Max flow rate: 400 cm/h (20°C, aqueous buffers, 10 x 300 mm column).
Chemical stability: Compatible with all standard aqueous buffers used for protein purification, e.g. 10mM HCl (pH2), 10mM NaOH (pH12), 0.1M sodium citrate-HCl (pH3), 6M guanidineHCl, 20% ethanol. Should not be stored at low pH for prolonged time.
pH stability (short term): 3 - 10
pH stability (cleaning): 2 - 12
Storage: +2 to +8 °C in 20 % ethanol
application:Affinity chromatography for purifying IgGs.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04
Dokumente - Protokolle - Downloads
Here you will find information and further literature on WorkBeads® Protein A. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.