WorkBeads® Protein A

WorkBeads Protein A


Order number: S5350.0005

Shipping: shipped at RT, store at +2°C to +8°C

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€268.29 *

Volume:

Please select the favoured pack size.

WorkBeads Protein A is a ready-to-use resin. No additional mixing or reagents are required. It... more
Product information "WorkBeads® Protein A"

WorkBeads Protein A is a ready-to-use resin. No additional mixing or reagents are required. It is not necessary to warm the product to room temperature before use. Protein A is a surface protein originally found in the cell wall of bacteria Staphylococcus aureus, Group G Streptococci and Peptostreptococcus magnus, respectively. Protein A and protein G bind with high affinity and specificity to Fc region of immunoglobulins from many of mammalian species, most notably IgGs. Unlike protein A and G, Protein L binds immunoglobulins through kappa light chain. These proteins have been usually used to purify, immobilize or detect immunoglobulins.

Advantages compared to competitor products:

- Higher dynamic binding capacity for monoclonal antibodies;
- Excellent recovery & high purity
- Stronger coupling chemistry; high pH stability and low leakage
- Reliable, reproducible and efficient

The product is supplied as a 50% suspension in 20% Ethanol. 10mL of the shipped product for example are equivalent to 5mL pure Resin plus 5mL solvent.

More products around "WorkBeads® Protein A"
Our comment on "WorkBeads® Protein A"
Low ligand leakage. Compatible with all standard aqueous buffers used for protein purification. pH stability: pH3–10 short term; pH2–12 for cleaning.
Specifications: Target substance: Polyclonal and monoclonal antibodies, bound via the... more
 

Technical Data:

Specifications:
Target substance: Polyclonal and monoclonal antibodies, bound via the Fc-region.
Matrix: Rigid, highly cross-linked agarose.
Average particle size (Dv50): 45µm.
50% suspension in 20% ethanol.
Ligand: Recombinant protein A expressed in E. coli using animal-free medium.
Dynamic binding capacity (DBC) of typically more than 40mg IgG per 1mL medium.
Recommended flow rate: 250 cm/h (aqueous buffers).
Max flow rate: 400 cm/h (20°C, aqueous buffers, 10 x 300 mm column).
Chemical stability: Compatible with all standard aqueous buffers used for protein purification, e.g. 10mM HCl (pH2), 10mM NaOH (pH12), 0.1M sodium citrate-HCl (pH3), 6M guanidineHCl, 20% ethanol. Should not be stored at low pH for prolonged time.
pH stability (short term): 3 - 10
pH stability (cleaning): 2 - 12
Storage:  +2 to +8 °C in 20 % ethanol

application:

Affinity chromatography for purifying IgGs.

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on WorkBeads® Protein A. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
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