CentriPure 5 columns for Purification of Proteins

CentriPure 5 columns are pre-hydrated gel filtration columns for protein purification and desalting. Process sample volume of 500µL. CentriPure 5 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.

The size exclusion cut-off for Zetadex-25 is 5 kDa for proteins. Proteins larger than 5 kDa in a sample volume of up to 650µL can be purified with an elution volume of 650 to 900µL.