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Rho1D4 Agarose

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  • High-quality products
  • Customized solutions
  • Personal contact
  • Fast service
  • Competent technical support +49(0)731-3608-123

€1,200.38*

Volume
Product number: S5384.0005
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!
Product information "Rho1D4 Agarose"

Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system.

Properties: 
- demonstrably suitable for membrane protein research 
- binding capacity of 3-4mg protein per mL resin 
- purifies protein with high specificity 
- gentle protein elution based on competitive binding 
- can be regenerated for reuse.

Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution.

Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Specifications:
Usage: Specific binding and purification of Rho1D4-tagged proteins
Binding: Specificity Affinity to Rho1D4-tagged (TETSQVAPA) proteins
Binding capacity: >3 mg/mL
Bead Ligand: anti-Rho1D4 antibody
Bead size: 100μm Matrix 6% cross-linked agarose
Recommended flow rates: 0.25 - 1 mL/min
Max. flow rates: 6 mL/min

Delivered as a 50 % suspension



2mL of a 50% agarose suspension (1mL final gel bed). Suspension in 20% ethanol to prevent microbial growth.


Applikation / Application:
Einheiten / Units:
Quelle / Source:
synthetic


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-17-03-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Protocols
Manuals
General Data 1
General Data 2
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed