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Rho1D4 MagBeads

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  • High-quality products
  • Customized solutions
  • Personal contact
  • Fast service
  • Competent technical support +49(0)731-3608-123

€1,280.10*

Volume
Product number: S5394.0005
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: on wet ice. Store at +2°C to +8°C. For laboratory usage only!
Product information "Rho1D4 MagBeads"

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. The rho1D4 purification system is based on the highly specific binding of the rho1D4 antibody to the rho1D4 epitope fused to proteins. This system has proven particularly effective with membrane proteins. Magnetic beads are useful for protein purification of dilute samples, and for pull-down experiments to determine protein-protein interactions. Elution of the protein can be done the rho1D4 peptide, which competitively binds to the rho1D4 antibody, thereby release the traget protein. The peptide can be purchased individually, or together with Genaxxon Rho1D4 MagBeads as part of a Starter Set. A 5% suspension is provided that binds up to 3mg of protein per mL settled beads.

Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Specifications:
Usage: Specific binding and purification of Rho1D4-tagged proteins
Binding: Specificity Affinity to Rho1D4-tagged (TETSQVAPA) proteins
Binding capacity: >3 mg/mL
Bead Ligand: anti-Rho1D4 antibody
Bead size: 30 μm
Matrix: 6% cross-linked agarose
Delivered as a 25 % suspension

Applikation / Application:
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Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-17-03-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Protocols
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General Data 2
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed