Total RNA Purification Mini Spin Kit

Total RNA Purification Kit from Genaxxon


Order number: S5304.0100

Shipping: Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€299.50 *

pack size:

Please select the favoured pack size.

Genaxxon´s Total RNA Purification Mini Spin kit is designed for the rapid and efficient... more
Product information "Total RNA Purification Mini Spin Kit"

Genaxxon´s Total RNA Purification Mini Spin kit is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) and 10E4-10E7 cultured cells! Comparable with Qiagen RNeasy Mini Kit (74104).

Genaxxon´s Total RNA Purification Mini Spin kit is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) and 10E4-10E7 cultured cells. The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Mini Spin kit utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed. Any RNases are inactivated. The three-step washing stage effectively removes impurities and enzyme inhibitors.

The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.

Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.

You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.

With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!

Please check also our DNA purification kits >.

More products around "Total RNA Purification Mini Spin Kit"
Our comment on "Total RNA Purification Mini Spin Kit"
230µg RNA binding capacity on-column DNase I digestion for faster purification fast and efficient RNA purification
Specifications: Purity of isolated RNA: A260/A280 = 1.7 - 1.9 Binding capacity of columns:... more
 

Technical Data:

Specifications:
Purity of isolated RNA: A260/A280 = 1.7 - 1.9
Binding capacity of columns: approximately 230µg RNA
for up to 30mg frozen or preserved tissue
or for 10E4 - 10E7 cells from cell culture.

application:

RNA purification for downstream applications as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc.

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on Total RNA Purification Mini Spin Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy

Isabella Syring, Richard Weiten, Tim Müller, Doris Schmidt, Susanne Steiner, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger

Oncol Lett. 2018 Sep; 16(3): 3013–3021. Published online 2018 Jun 25. doi: 10.3892/ol.2018.9014

PMCID: PMC6096071

Comprehensive analysis of the transcriptional profile of the Mediator complex across human cancer types

Isabella Syring, Niklas Klümper, Anne Offermann, Martin Braun, Mario Deng, Diana Boehm, Angela Queisser, Anne von Mässenhausen, Johannes Brägelmann, Wenzel Vogel, Doris Schmidt, Michael Majores, Anne Schindler, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger, Zaki Shaikhibrahim, Sven Perner

Oncotarget. 2016 Apr 26; 7(17): 23043–23055.  Published online 2016 Mar 30. doi: 10.18632/oncotarget.8469

PMCID: PMC5029609