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Total RNA Purification Mini Spin Kit PLUS with ceramic beads for the rapid and efficient isolation and extraction of high quality RNA from 1-30mg of tissue (fresh or frozen) and 10E4 - 10E7 cultured cells. Additionally to our kit S5304 > the Mini Spin Total RNA Purification Kit PLUS contains homogenization tubes with ceramic beads for desintegration of cells. The isolation protocol and buffer formulations were optimised for high isolation and extraction efficiency and purity of RNA. The Mini Spin Total RNA Purification kit utilizes spin mini columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts.
The isolation procedure with the Mini Spin Total RNA Purification kit consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed with guanidine thiocyanate and detergents. Any RNases are inactivated by guanidine thiocyanate and β-mercaptoethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors.
The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc..
Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.
You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.
With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!
Please check also our DNA purification kits >.
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230µg RNA binding capacity on-column DNase I digestion for faster purification fast and efficient RNA purification
Purity of isolated RNA: A260/A280 = 1.7 - 1.9
Binding capacity of columns: approximately 230µg RNA
for up to 30mg frozen or preserved tissue
or for 10E4 - 10E7 cells from cell culture.
application:RNA purification for downstream applications as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Total RNA Purification Mini Spin Kit PLUS. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.