Order number: S5305.0050Shipping: shipped at RT, store at +2°C to +8°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
miRNA Purification Mini Spin Kit for rapid and unbiased, phenol-free extraction of highly enriched small RNA (<200nt) with superior yield and purity, from a wide range of cells, tissues and blood. It is possible to extract high quality large RNA (>200nt) in parallel from the same sample.
The kit contains 3 different types of purification columns: 1. for DNA removal; 2. for purification of large RNA and 3. for purification of small RNA (tRNA, 5S rRNA, 5.8S rRNA and regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA)).
RNA and DNA can be isolated and purified from 1-10mg of tissue (fresh or frozen) or 10E4 - 10E6 cultured cells.
The use of this kit results in efficient removal of large RNA molecules such as 28S rRNA, 18S rRNA, and mRNA that can inhibit expression analysis of small RNA molecules however the separate purification and isolation of these large RNAs is also possible using this kit.
Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.
You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.
With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!
Please check also our DNA purification kits >.
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- microRNA transfer
- Further products by Genaxxon bioscience
separates regulatory RNA (miRNA; siRNA) from "large" RNA like 28S RNA, etc. parallel isolation of small RNA, large RNA and DNA
Kit for isolation of miRNA but also for DNA!
- Purity of isolated miRNA: A260/A280 = 1.9 - 2.1.
- Binding capacity: approx. 90µg RNA.
- fresh or frozen tissue (stored at -80°C): 1–10 mg
- tissue preserved in RNase inactivating buffers: 1–10 mg
- cell culture: 10E4 - 10E6 cells from cell culture.
application:for purification of high quality miRNA or siRNA.
Sicherheits Hinweise / SafetyH-Sätze: H315, H302+H312+H332, H225, H319, H336
GHS-Symbole: GHS02, GHS05, GHS07
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on miRNA Purification Mini Spin Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.