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RNA Purification from Bacteria and Yeast Kit for rapid and efficient phenol free RNA Extraction and Purification of high quality RNA from bacteria or yeast cultures or frozen cells. Up to 1 x 10E9 bacteria cells or up to 5 x 10E7 yeast cells.
The isolation protocol and buffer formulations were optimized for high isolation efficiency and purity of total RNA. This kit uses spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. In the first step of the isolation procedure the cell walls are disintegrated and the membranes and proteins are degraded. Any RNases are inactivated by guanidine thiocyanate and β-mercaptoethanol. The homogenate is separated from the undigested cell remains by centrifugation and on the Homogenizing Column. The RNA is bound to the Purification Column membrane by addition of ethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water (pH 7.0-9.0) and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.
Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.
You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.
With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!
Please check also our DNA purification kits >.
Purity of isolated miRNA: A260/A280 = 1.9 - 2.1.
Binding capacity: approx. 90µg RNA.
YIELD: depends on the sample material amount and type.
- up to 60μg RNA from bacterial culture
- up to 30μg RNA from yeast culture
application:for purification of high quality RNA from bacteria and Yeast
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on RNA Purification from Bacteria and Yeast Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.