Order number: S5318.0100Shipping: shipped at RT, stored at +2°C to +8°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
GENAzol is a ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA and proteins from biological samples. With this single phase solution of phenol and guanidine isothiocyanate, RNA, DNA and proteins in different fractions from cell and tissue samples, e.g. isolated from humans, animals, plants, yeasts, bacteria or viruses (usually within an hour).
Properties of GENAzol:
- Parallel isolation of RNA, DNA and protein from the same sample
- Superior suitability for lysis even with difficult samples
- Optimized formulations and protocols for tissues, cells, serum, viruses and bacteria.
Reliable purification of RNA from samples of different volumes and sources
GENAzol is also suitable for small amounts of tissue (50–100mg) and cells (5× 10E6) as well as for larger amounts of cells (>10E7) and contains protocols for the purification of RNA from human, animal, plant or bacterial Rehearse. GENAzol maintains the integrity of the RNA due to the very effective inhibition of RNase activity when cells and components are dissolved during sample homogenization. GENAzol enables the simultaneous processing of a large number of samples. The entire isolation step is usually completed in 1 hour. The total RNA isolated with GENAzol is free from protein and DNA contamination.
Procedure for the isolation of RNA, DNA and proteins
GENAzol enables sequential precipitation of RNA, DNA and proteins from a single sample. After homogenizing the sample with GENAzol, chloroform is added and the homogenate separates into a clear aqueous top layer (which contains RNA), a boundary layer and a organic bottom layer (with DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA precipitation from the organic boundary layer takes place with ethanol. Finally, the protein from the phenol-ethanol supernatant is precipitated with isopropanol. Precipitated RNA, DNA or protein is washed to remove impurities and then resuspended for use in subsequent applications.
- Quick isolation of high-quality total RNA, DNA and/or protein from a single sample - Performs well with large or small amounts of tissue or cells - Ready-to-use solution
Isolation of RNA, DNA and protein from the same sample
1mL GENAzol for 100mg tissue or up to 1x 10E7 cells
Purity: A260/A280 ratio: 1.7 – 1.9.
Time required: 5 - 10 min.
application:- Purified RNA is ideal for any downstream application such as RT-PCR, in vitro translation, northern blotting, RNase protection assays or dot blot hybridization - Purified DNA can be used for PCR and Southern blotting - Purified protein can be used for western blotting
Sicherheits Hinweise / SafetyH-Sätze: H301, H312, H331, H314, H318, H341, H373, H411
GHS-Symbole: GHS05, GHS06, GHS08
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on GENAzol - RNA Purification solution. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.