Viral RNA Purification Kit

Viral RNA Purification Kit


Order number: S5301.0250

Shipping: shipped and stored at RT

Delivery time 45 Workdays

€725.00 *

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This Viral RNA and DNA Preparation Kit is designed for rapid and effective isolation of RNA and... more
Product information "Viral RNA Purification Kit"

This Viral RNA and DNA Preparation Kit is designed for rapid and effective isolation of RNA and DNA from a variety of pathogen organisms such as virus or bacteria. Samples can be fresh or frozen plasma/blood (treated with anticoagulants EDTA, Citrate, or ACD), serum, other cell-free body fluids or pathogen-infected tissue. The Kit is specifically designed to isolate high-quality nucleic acids using low elution volumes and allowing sensitive downstream analysis including quantitative PCR and RT-PCR. The purified RNA/DNA is free of proteins and nucleases. The Viral RNA and DNA Preparation Kit uses a lysis buffer including chaotropic salts to inactivate RNases/DNases and advanced silica-gel membrane technology for fast purification of intact RNA/DNA.

The preparation procedure is optimized to give reproducible results within 30 min.

Preparation procedure:
Preparation from plasma, serum, urine, cell-culture supernantant, cell-free fluid or virus infected tissue
Transfer 150µL plasma, serum, urine, cell-culture supernatant, cell-free fluid or virus infected tissue into a 1.5mL microtube.
Note: Adjust lower sample volumes with PBS Buffer to 150µL. Samples of larger volumes (up to 300µL) can easily be scaled up but may require larger tubes for the lysis procedure.
Add 300µL (2 amounts of sample volume) of Lysis Buffer.
Vortex for 15 sec.
Incubate at room temperature (20°C - 25°C) for 10 min.
Add 300µL (2 amounts of sample volume) Binding Buffer and mix well by gently vortexing.

Preparation from nasal or throat swabs
Transfer 150µL PBS Buffer into a 1.5 ml microtube.
Add 300µL of Lysis Buffer.
Cut off the cutton tip with the collected nasal or throat cells and place it in the micro tube.
Close the tube and vortex for 15 sec.
Incubate at room temperature (20°C - 25°C) for 10 min.
Add 300µL Binding Buffer and mix well by gently vortexing.

Purification procedure
Column Loading

Place a Spin Column into a provided 2mL collection tube.
Load lysate on the column and centrifuge at 13,000 g for 1 min.
Note: The maximum volume of the column reservoirs 800μL. For larger sample volumes discard the flow-through and load the column / spin again.
Discard the flow-through in the collection tube and place the column back in the same tube.

Column Washing
Add 500µL Washing Buffer A to the Spin Column and centrifuge at 13,000 g for 1 min.
Discard the flow-through in the collection tube and place the Spin Column back in the same tube.
Add 500µL Washing Buffer B to the Spin Column and centrifuge at 13,000 g for 1 min.
Note: Before using Washing Buffer B for the first time add ethanol as indicated on the bottle.
Discard the flow-through in the collection tube and place the Spin Column back in the same tube.
Centrifuge at 13,000 g for 1 min.
Note: It is important to dry the membrane since residual ethanol may interfere with downstream reactions.

Elution
Place the Spin Column into a new 1.5 ml microtube (not provided).
Add 30-60µL Elution Buffer directly onto the membrane of the spin column.
Note: Avoid touching membrane with the pipet tip.
Incubate at room temperature for 1 min.
Centrifuge at 13,000 g for 1 min.
The eluted RNA and/or DNA is ready for down-stream processing or for storage at -80°C.
Use 2-5µL as template for PCR or RT-PCR.

Examples of downstream application: RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RNA sequencing, micro arrays.

Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.

You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.

With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!

More products around "Viral RNA Purification Kit"
Specifications: Purity of isolated RNA: A260/A280 = 1.9 - 2.1 Binding capacity of columns:... more
 

Technical Data:

Specifications:
Purity of isolated RNA: A260/A280 = 1.9 - 2.1
Binding capacity of columns: approximately 120µg RNA
Time for purification: about 10-12 minutes

application:

RNA purification for downstream applications as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc.

Sicherheits Hinweise / Safety

H-Sätze: H225, H314, H315, H319, H335, H412
GHS-Symbole: GHS02, GHS05, GHS07,

Klassifizierungen / Classification

eclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on Viral RNA Purification Kit. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy

Isabella Syring, Richard Weiten, Tim Müller, Doris Schmidt, Susanne Steiner, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger

Oncol Lett. 2018 Sep; 16(3): 3013–3021. Published online 2018 Jun 25. doi: 10.3892/ol.2018.9014

PMCID: PMC6096071

Comprehensive analysis of the transcriptional profile of the Mediator complex across human cancer types

Isabella Syring, Niklas Klümper, Anne Offermann, Martin Braun, Mario Deng, Diana Boehm, Angela Queisser, Anne von Mässenhausen, Johannes Brägelmann, Wenzel Vogel, Doris Schmidt, Michael Majores, Anne Schindler, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger, Zaki Shaikhibrahim, Sven Perner

Oncotarget. 2016 Apr 26; 7(17): 23043–23055.  Published online 2016 Mar 30. doi: 10.18632/oncotarget.8469

PMCID: PMC5029609