Total RNA Purification Kit - Tissue
Advantages at a glance
- Purity of isolated RNA: A260/A280 = 1.7 - 1.9
- Binding capacity of columns: approximately 230µg RNA
- For up to 30mg fresh or frozen tissue
- For up to 25mg Paraffin embedded tissue
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
Genaxxon´s Total RNA Purification Kit - Tissue is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) or up to 25mg Paraffin embedded tissue.
The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed. Any RNases are inactivated. The three-step washing stage effectively removes impurities and enzyme inhibitors.
The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.
Genaxxon offers also ceramic beads > for rapid cell disintegration and other RNA purification kits >.
With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.
Here you will find further Purification kits >.
Specifications:
Purity of isolated RNA: A260/A280 = 1.7 - 1.9
Binding capacity of columns: approximately 230µg RNA
For up to 30mg fresh or frozen tissue
For up to 25mg Paraffin embedded tissue
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-04-05
Documents:
ProtocolsManuals
General Data 1
General Data 3
General Data 4
Source: NCBI PubMed
The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy
Isabella Syring, Richard Weiten, Tim Müller, Doris Schmidt, Susanne Steiner, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger
Oncol Lett. 2018 Sep; 16(3): 3013–3021. Published online 2018 Jun 25. doi: 10.3892/ol.2018.9014
PMCID: PMC6096071
Isabella Syring, Niklas Klümper, Anne Offermann, Martin Braun, Mario Deng, Diana Boehm, Angela Queisser, Anne von Mässenhausen, Johannes Brägelmann, Wenzel Vogel, Doris Schmidt, Michael Majores, Anne Schindler, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger, Zaki Shaikhibrahim, Sven Perner
Oncotarget. 2016 Apr 26; 7(17): 23043–23055. Published online 2016 Mar 30. doi: 10.18632/oncotarget.8469
PMCID: PMC5029609
