SNP - Allele Specific Discrimination, Genotyping and Gene Expression Analysis

For successful allele specific discrimination, genotyping of SNPs and the detection of point mutations, e.g. in cancer related genes. SNP Pol DNA Polymerase > is a highly selective DNA polymerase variant, that has been specially developed designed for all approaches in which allele specific discrimination or high single nucleotide discrimination is required. For instance in allele specific PCRs (ASA), primer extensions or methylation-specific PCRs (MSP).

Mutant alleles can be distinguished exactly from wild-type alleles - without sequencing since the polymerase does not amplify in the case of a mismatch.

SNP Pol DNA Polymerase > efficiently discriminates primers hich have a mismatch at the 3'-end. The polymerase amplifies only if the primers fit 100% at the 3'-end. There´s no amplification if primers have even a single base exchange (point mutation) directly at the 3'-end.
Whereas many DNA polymerases tolerate mismatched primer-template complexes, SNP Pol DNA polymerase efficiently discriminates those and only produces specific amplicons in case of perfectly matched primer pairs. This makes SNP Pol DNA polymerase very useful for SNP detections, HLA genotyping or the analysis of single CpG methylation sites!

The uniquely engineered polymerase is also available as SNP PolTaq variant > which has a 5'-3'-nuclease activity and therefore can be used for hydrolysis probe-based assays as Molecular beacons or TaqMan® probes in probe qPCR.

Frequently asked questions (FAQs) >

Online form for your SNP Pol test sample >