Bluo-Gal / 5-Bromo-3-indolyl-beta-D-galactopyranoside
Sipariş numarası: M3225.0100
Shipping: store at -20°C in the dark; keep away from lightCAS-Nr: 97753-82-7
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5-Bromo-3-indolyl-β-D-galactopyranosid (Bluo-Gal) ist used as a substrat of β-Galactosidase. It is a chromogenic substrate suitable for identification of lacZ+ bacterial colonies. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Principle
When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
Technische Daten:
Specifications:
Assay: min. 98.0% (HPLC)
Appearance: white to white-off powder
C14H16BrNO6
MW = 374.18 g/mol
Soluble in: DMF
Applikation:
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color. Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.Quelle
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
CAS-Nr: 97753-82-7eclass-Nr: 32-16-04-90
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