BCIP, molecular biology grade



Sipariş numarası: M3196.0500

Shipping: shipped at RT, stored at -20°C
CAS-Nr: 6578-06-9

Teslim süresi yaklaşık. 5 iş günü

77,67 € *

Weight:

Please select the favoured pack size.

BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for... daha fazla
Ürün bilgileri "BCIP, molecular biology grade"

BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase, used in combination with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development.

Applications:
When alkaline phosphatase conjµgates are used for detection, BCIP is recommended for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots.

It is recommended to prepare a 50mg/mL stock solution in DMF.
Protocol for Preparation of BCIP/NBT Substrate Solution:
Add the following components
- 10mL of the Alkaline Phosphatase (AP) buffer (100mM Tris-HCl (pH9.5), 100mM NaCl and 10mM MgCl2)
- 33µL BCIP (50mg/mL in DMF)
- 44µL NBT (75mg/mL in 70% DMF).

NOTE: Avoid exposure to light - Prepare fresh developing solution and use within an hour - BCIP and NBT solutions in DMF do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.

İlgili bağlantılar "BCIP, molecular biology grade"
Specifications Assay: min. 98% (HPLC) pH (5% in water): 1.0-2.0 Solubility (water, 20°C): 582... daha fazla
 

Technische Daten:

Specifications
Assay: min. 98% (HPLC)
pH (5% in water): 1.0-2.0
Solubility (water, 20°C): 582 g/L
MP = 120°C
Chloride: max. 0.001%
Pb: max. 0.005%
Free acid: max. 0.1%
C8H6BrClNO4P x C7H9N
MW = 433.64 g/mol

Quelle

synthetic

Sicherheits Hinweise / Safety

H-Sätze: H315, H319, H335
GHS-Symbole: GHS07

Klassifizierungen / Classification

EC-Nr: 229-506-1
CAS-Nr: 6578-06-9
eclass-Nr: 32-16-04-90
Dokumente - Protokolle - Downloads daha fazla


Dokumente - Protokolle - Downloads

Hier finden Sie Informationen und weiterführende Literatur zu BCIP, molecular biology grade. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.