Sipariş numarası: M3005.0100Shipping: Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
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LyoPlex Multiplex PCR master mix for fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR. This specially optimized and lyophilized master mix promotes fast multiplex real-time PCR. The combination of the specially designed hot start DNA polymerase together with a unique PCR buffer system as a lyophilized ready-to-use master mix ensures highly sensitive qPCR together with a very convenient storage at room temperature.
Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy.
- Amplification of multiple targets in a single tube (tested for up to 5 targets)
- All-in-one master mix for convenient multiplexing
- High specificity, sensitivity and product yield
- Easy reaction setup at room temperature
- stable for 24 months at room temperature
The Multiplex PCR master mix is a premixed, ready-to-use solution containing our highly specific chemically modified Taq DNA Polymerase, highly pure dNTPs, MgCl2, additives and a reaction buffer at optimal concentrations which enable reliable multiplex PCR, e.g. microsatellite, clonality or gene rearrangement analysis. Just add your primers and template DNA.
The Multiplex HS Master mix 2X is shipped in aliquots of 1.25mL.
realtime master mixes for your realtime PCR experiments can be found here >.
Examples of Multiplex applications:
F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002.
Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.
The Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. - Amplification of multiple targets in a single tube (tested for up to 5 targets) - All-in-one master mix for convenient multiplexing - High specificity, sensitivity and product yield - Easy reaction setup at room temperature - stable at room temperature
2-time ready-to-use lyophilised Multiplex master mix with an optimized modified Taq DNA polymerase without green fluorescent dye and without ROX™.
Aliquot size: 250µL.
The lyophilized realtime PCR master mix is stable for at least 24 months at room temperature.
Applikation:Multiplex PCR for Microsatellite analysis clonality analysis gene rearrangement analysis
Einheitendefinition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-05-02
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