Penicillin-Streptomycin (10000 U/mL)
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Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Penicillin-Streptomycin 10000 U/mL: Penicillin G aus Penicillium notatum und Streptomycin aus Streptomyces griseus verhindern mit ihrer kombinierten Wirkung (Pen-Strep) gegen grampositive und gramnegative Bakterien eine bakterielle Kontamination von Zellkulturen. Penicillin G wirkt einerseits direkt, indem es den Neuaufbau der bakteriellen Zellwand stört, sowie indirekt, indem es die Freisetzung von Enzymen auslöst, die die Zellwand weiter verändern. Streptomycin wirkt, indem es sich an die 30S-Untereinheit des bakteriellen Ribosoms anlagert, was zu einer Hemmung der Protein-Synthese und zum anschließenden Absterben der Bakterien führt.
Penicillin G Kaliumsalz ist in Wasser gut löslich. Bei +4°C oder Raumtemperatur ist die Trockensubstanz ca. 5 Jahre stabil. Die gepufferte Lösung (pH4,5-7) verliert bei +4°C nach 20 Tagen 5% an Aktivität und nach 60 Tagen ca. 20%. Bei Raumtemperatur ist bereits nach 48 Stunden ein 5%iger, nach 7 Tagen ein 10%iger und nach 14 Tagen ein 20 - 50%iger Aktivitätsverlust zu verzeichnen. In einer ungepufferten Lösung ist die Stabilität erheblich geringer (+4°C ca. 3-7 Tage).
Pen-Strep: Penicillin G und Streptomycin gegen bakterielle Kontamination von Zellkulturen.
Genaxxon bioscience bietet neben Pen-Strep eine breite Palette an Antibiotika und Antimykotika als Pulver und in flüssiger Form an, z.B. Geneticin G418, Kanamycin oder Gentamycinsulfat.
Die Mindestbestellmenge beträgt 5 x M3140.0100 (100mL).
Specifications:
Penicillin G content: 9,000 – 11,000 U/mL
Streptomycin content: 9 – 11 mg/mL
0.9% NaCl (physiological NaCl solution)
pH: 6.0 – 7.0
Osmolality: 280 – 400 mOsmol/kg
Endotoxin: <1 EU/mL
Bioburden: 0 CFU/mL
Shelf-life at 37°C: 5 days
sterile filtered solution for cell culture applications
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-19-90-90
Dokumente:
SicherheitsdatenblätterZertifikate
Produktbeschreibung
Kategorienliste
Quelle: NCBI PubMed
CoCl2 -triggered pseudohypoxic stress induces proteasomal degradation of SIRT4 via polyubiquitination of lysines K78 and K299.
Nils Hampel 1, Jacqueline Georgy, Mehrnaz Mehrabipour, Alexander Lang, Isabell Lehmkuhl, Jürgen Scheller, Mohammad R. Ahmadian, Doreen M. Floss, Roland P. Piekorz
FEBS Open Bio. 2023 Dec;13(12):2187-2199.
doi: 10.1002/2211-5463.13715.
PMCID: PMC10699113.
Multimerization strategies for efficient production and purification of highly active synthetic cytokine receptor ligands.
Sofie Mossner, Hoang T Phan, Saskia Triller, Jens M Moll 1, Udo Conrad, Jürgen Scheller
PLoS One. 2020 Apr 1;15(4):e0230804.
doi: 10.1371/journal.pone.0230804.
PMCID: PMC7112226.
Synthetic interleukin 22 (IL-22) signaling reveals biological activity of homodimeric IL-10 receptor 2 and functional cross-talk with the IL-6 receptor gp130.
Sofie Mossner, Marcus Kuchner, Nastaran Fazel Modares, Birgit Knebel, Hadi Al-Hasani, Doreen M Floss, Jürgen Scheller
J Biol Chem. 2020 Aug 28;295(35):12378-12397.
doi: 10.1074/jbc.RA120.013927.
PMCID: PMC7458808.
Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors.
Alessandra Esch, Anna Masiarz, Sofie Mossner, Jens M Moll, Joachim Grötzinger, Jutta Schröder, Jürgen Scheller, Doreen M Floss
J Biol Chem. 2020 Jul 24;295(30):10478-10492.
doi: 10.1074/jbc.RA120.013935.
PMCID: PMC7383385.
bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling
Fereshteh Haghighi, Julia Dahlmann, Saeideh Nakhaei-Rad, Alexander Lang, Ingo Kutschka, Martin Zenker, George Kensah, Roland P. Piekorz, Mohammad Reza Ahmadian
Cell Commun Signal. 2018; 16: 96.
doi: 10.1186/s12964-018-0307-1
PMCID: PMC6282345
IL-23R Signaling Plays No Role in Myocardial Infarction
Erika Engelowski, Nastaran Fazel Modares, Simone Gorressen, Pascal Bouvain, Dominik Semmler, Christina Alter, Zhaoping Ding, Ulrich Flögel, Jürgen Schrader, Haifeng Xu, Philipp A. Lang, Jens Fischer, Doreen M. Floss, Jürgen Scheller
Sci Rep. 2018; 8: 17078.
doi: 10.1038/s41598-018-35188-8
PMCID: PMC6244091
Prevention of bacterial colonization on non-thermal atmospheric plasma treated surgical sutures for control and prevention of surgical site infections
Utku Kürşat Ercan, Fatma İbiş, Caner Dikyol, Nesrin Horzum, Ozan Karaman, Çağla Yıldırım, Elif Çukur, Emine Afra Demirci
PLoS One. 2018; 13(9): e0202703.
doi: 10.1371/journal.pone.0202703
PMCID: PMC6124751
Synthetic cytokine receptors transmit biological signals using artificial ligands
Erika Engelowski, Artur Schneider, Manuel Franke, Haifeng Xu, Ramona Clemen, Alexander Lang, Paul Baran, Christian Binsch, Birgit Knebel, Hadi Al-Hasani, Jens M. Moll, Doreen M. Floß, Philipp A. Lang, Jürgen Scheller
Nat Commun. 2018; 9: 2034.
doi: 10.1038/s41467-018-04454-8
PMCID: PMC5964073
IL-6/IL-12 Cytokine Receptor Shuffling of Extra- and Intracellular Domains Reveals Canonical STAT Activation via Synthetic IL-35 and IL-39 Signaling
D. M. Floss, M. Schönberg, M. Franke, F. C. Horstmeier, E. Engelowski, A. Schneider, E. M. Rosenfeldt, J. Scheller
Sci Rep. 2017; 7: 15172.
doi: 10.1038/s41598-017-15173-3
PMCID: PMC5680241
Synthetic Deletion of the Interleukin 23 Receptor (IL-23R) Stalk Region Led to Autonomous IL-23R Homodimerization and Activation
Thorben M. Hummel, Theresa Ackfeld, Marco Schönberg, Gregor Ciupka, Falk Schulz, Anne Oberdoerster, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss
Mol Cell Biol. 2017 Sep 1; 37(17): e00014-17.
doi: 10.1128/MCB.00014-17
PMCID: PMC5559674
Defining the functional binding sites of interleukin 12 receptor β1 and interleukin 23 receptor to Janus kinases
Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl, Heike Hermanns, Jürgen Scheller
Mol Biol Cell. 2016 Jul 15; 27(14): 2301–2316.
doi: 10.1091/mbc.E14-12-1645
PMCID: PMC4945146
A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1
Rene Geissler, Alfred Simkin, Doreen Floss, Ravi Patel, Elizabeth A. Fogarty, Jürgen Scheller, Andrew Grimson
Genes Dev. 2016 May 1; 30(9): 1070–1085.
doi: 10.1101/gad.277392.116
PMCID: PMC4863738
Human and Murine Interleukin 23 Receptors Are Novel Substrates for A Disintegrin and Metalloproteases ADAM10 and ADAM17.
Manuel Franke, Jutta Schröder, Niloufar Monhasery, Theresa Ackfeld, Thorben M Hummel, Björn Rabe, Christoph Garbers, Christoph Becker-Pauly, Doreen M Floss, Jürgen Scheller
J Biol Chem. 2016 May 13;291(20):10551-61.
doi: 10.1074/jbc.M115.710541.
PMCID: PMC4865905.
Claudia J. Krause, Oliver Popp, Nanthakumar Thirunarayanan, Gunnar Dittmar, Martin Lipp, Gerd Müller
Oncotarget. 2016 Mar 1; 7(9): 10414–10432.
doi: 10.18632/oncotarget.7248
PMCID: PMC4891129
Sertoli cells have a functional NALP3 inflammasome that can modulate autophagy and cytokine production
Soren Hayrabedyan, Krassimira Todorova, Asma Jabeen, Gergana Metodieva, Stavri Toshkov, Metodi V. Metodiev, Milcho Mincheff, Nelson Fernández
Sci Rep. 2016; 6: 18896.
doi: 10.1038/srep18896
PMCID: PMC4705529
Sec14-nodulin proteins and the patterning of phosphoinositide landmarks for developmental control of membrane morphogenesis
Ratna Ghosh, Marília K. F. de Campos, Jin Huang, Seong K. Huh, Adam Orlowski, Yuan Yang, Ashutosh Tripathi, Aaron Nile, Hsin-Chieh Lee, Marek Dynowski, Helen Schäfer, Tomasz Róg, Marta G. Lete, Hasna Ahyayauch, Alicia Alonso, Ilpo Vattulainen, Tatyana I. Igumenova, Gabriel Schaaf, Vytas A. Bankaitis
Mol Biol Cell. 2015 May 1; 26(9): 1764–1781.
doi: 10.1091/mbc.E14-10-1475
PMCID: PMC4436786
Non-Canonical Interleukin 23 Receptor Complex Assembly: P40 PROTEIN RECRUITS INTERLEUKIN 12 RECEPTOR β1 VIA SITE II AND INDUCES P19/INTERLEUKIN 23 RECEPTOR INTERACTION VIA SITE III
Jutta Schröder, Jens M. Moll, Paul Baran, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss
J Biol Chem. 2015 Jan 2; 290(1): 359–370.
doi: 10.1074/jbc.M114.617597
PMCID: PMC4281739
Alternative Intronic Polyadenylation Generates the Interleukin-6 Trans-signaling Inhibitor sgp130-E10
Jan Sommer, Christoph Garbers, Janina Wolf, Ahmad Trad, Jens M. Moll, Markus Sack, Rainer Fischer, Joachim Grötzinger, Georg H. Waetzig, Doreen M. Floss, Jürgen Scheller
J Biol Chem. 2014 Aug 8; 289(32): 22140–22150.
doi: 10.1074/jbc.M114.560938
PMCID: PMC4139227
Identification of autophagy as a longevity-assurance mechanism in the aging model Podospora anserina
Laura Knuppertz, Andrea Hamann, Francesco Pampaloni, Ernst Stelzer, Heinz D Osiewacz
Autophagy. 2014 May; 10(5): 822–834.
doi: 10.4161/auto.28148
PMCID: PMC5119060
PKCβ Phosphorylates PI3Kγ to Activate It and Release It from GPCR Control
Romy Walser, John E. Burke, Elena Gogvadze, Thomas Bohnacker, Xuxiao Zhang, Daniel Hess, Peter Küenzi, Michael Leitges, Emilio Hirsch, Roger L. Williams, Muriel Laffargue, Matthias P. Wymann
PLoS Biol. 2013 Jun; 11(6): e1001587.
doi: 10.1371/journal.pbio.1001587
PMCID: PMC3692425
Identification of Canonical Tyrosine-dependent and Non-canonical Tyrosine-independent STAT3 Activation Sites in the Intracellular Domain of the Interleukin 23 Receptor
Doreen M. Floss, Simone Mrotzek, Tobias Klöcker, Jutta Schröder, Joachim Grötzinger, Stefan Rose-John, Jürgen Scheller
J Biol Chem. 2013 Jul 5; 288(27): 19386–19400.
doi: 10.1074/jbc.M112.432153
PMCID: PMC3707643
Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663
Orwah Saleh, Katrin Flinspach, Lucia Westrich, Andreas Kulik, Bertolt Gust, Hans-Peter Fiedler, Lutz Heide Beilstein
J Org Chem. 2012; 8: 501–513.
doi: 10.3762/bjoc.8.57
PMCID: PMC3326630
The biosynthetic genes for prenylated phenazines are located at two different chromosomal loci of Streptomyces cinnamonensis DSM 1042
Kerstin Seeger, Katrin Flinspach, Elisa Haug‐Schifferdecker, Andreas Kulik, Bertolt Gust, Hans‐Peter Fiedler, Lutz Heide
Microb Biotechnol. 2011 Mar; 4(2): 252–262.
doi: 10.1111/j.1751-7915.2010.00234.x
PMCID: PMC3818865
Aromatic Prenylation in Phenazine Biosynthesis: DIHYDROPHENAZINE-1-CARBOXYLATE DIMETHYLALLYLTRANSFERASE FROM STREPTOMYCES ANULATUS
Orwah Saleh, Bertolt Gust, Björn Boll, Hans-Peter Fiedler, Lutz Heide
J Biol Chem. 2009 May 22; 284(21): 14439–14447.
doi: 10.1074/jbc.M901312200
PMCID: PMC2682892
Äquivalent/Alternative zu: Biochrom Cat.-no. A 2213, oder zu Bio&Sell Nr. BS.A2213.
Change in colour of the Pen/Strep solution:
Normally this change in colour does not mean that you can not use the solution anymore. There are different reasons for it.
1. Chemical reaction of Penicillin and oxygen which will form a substance that absorbs light to get a pale yellow colour. But this does not mean that the majority of the Penicillin is degraded. THere is still enough antibiotic in the solution.
2. The other point is, sometimes, that the Streptomycin source does still contain some pigments from the strain from which Streptomycin is isolated. These pigments may also undergo a reaction with oxygen that turns them more yellowish.
In any case you feel not good about the yellowish colour you should discard it, at this will be the only possibility to have no uncertainty for explanations of further results.
