Exosome Ultra
Vorteile im Überblick
- ready-to use
- lyophilized
- batch to batch consistency
- certified product
Lieferzeit 10 Tage
Shipment: not cooled. Store at -20°C. For laboratory usage only!
Exosome Ultra sind lyophilisierte Exosomen, die als Referenzmaterial/Standard für Ihre Exosomenforschung benutzt werden können. Lösen Sie die lyophilisierten Exosomen zum sofortigen Gebrauch einfach in sterilem Wasser auf. Herkunft: Menschliche MSC.
Exosomen sind kleine, nanoskalige extrazelluläre Vesikel (membranumhüllte Partikel von 30-120 nm), die von Endosomen abstammen und kontinuierlich von Zellen ausgeschieden werden. Exosomen sind unter anderem für die Zell-Zell-Kommunikation (Cell Signaling) von großer Bedeutung und können äußerst wertvolle Informationen über die Zusammensetzung von Proteinen, Lipiden, Metaboliten und Nukleinsäuren der ursprünglichen Mutterzelle liefern. Die Untersuchung des Inhalts der Exosomen kann Informationen über die Zellgesundheit, den allgemeinen Krankheitsstatus und mehr liefern. Daher wird von immer mehr Arbeitsgruppen untersucht, inwieweit Exosomen für diagnostische Zwecke eingesetzt werden können. Zumal nachgewiesen wurde, dass Exosomen bei Krebs, neurodegenerativen Erkrankungen und Herz-Kreislauf-Erkrankungen eine Rolle spielen.
Low Yield of exosomes can have different reasons of which some are listed here:
Exosome amount or concentration: Use samples contain more exosomes, e.g. concentrate samples or increase sample volume if possible.
Storage: Exosomes are very susceptible to repeated freeze-thaw cycles or to high storage temperatues. Always keep exosomes at -80°C for long term storage and do not allow repeated freeze-thaw cycles.
Kit related issues: Always centrifuge your sample at 16000xg before filtering it to get rid of the cell debris and to avoid clogging the filter membrane in the next step. Filter the centrifuged sample through a filter with a pore size between 0.8µm and 0.2µm.
If you use magnetic beads for further purification, the proportion of reagent volume is crucial for optimal efficiency. For this do not use less beads (less bead volume) than recommended and always use the recommended amount of reagent for incubation and washing. Incubation mode and elution intensity should be carried out in strict accordance with the instructions.
Sometimes the incubation time between samples and magnetic beads is too short. In such a case a prolonged incubation time can increase the exosome amount isolated as can an increased vortex intensity and vortex time.
- Use Gentle Isolation Conditions: Employ low temperatures and mild centrifugal forces during the isolation process. Avoid high-speed centrifugation steps that could cause exosome rupture.
- Optimize Buffers: Use buffers that protect the structural integrity of exosomes, such as phosphate-buffered saline (PBS) or Tris-buffered saline (TBS). Ensure the pH and osmolarity of the buffers are suitable for exosome stability.
- Proper Storage Conditions: Store exosomes at low temperatures, preferably -80°C, to prevent degradation. Avoid repeated freeze-thaw cycles as they can damage the exosomal membrane.
- Quality Control Assessments: Regularly perform quality control assessments such as transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. These assessments help check the morphology, size distribution, and protein markers of the exosomes to ensure their integrity.
Different isolation methods have varying capabilities to remove contaminants. For instance, immunocapture can use specific antibodies to bind exosomes, thereby reducing contamination from other components. Size exclusion chromatography (SEC) can also effectively separate exosomes from smaller particles.
- Strict Protocol Adherence: Maintain a clean work environment and use sterile techniques to avoid cross-contamination during the process. Use disposable centrifuge tubes and filters, and ensure all equipment is cleaned and calibrated regularly.
- Using the Right Filter Membrane: Ensure that the final filtration step is carried out in a clean bench. Use filter membranes with pore sizes no larger than 0.22 µm to effectively remove microorganisms.
- Using Specialized Kits and Reagents: Utilize specialized kits and reagents that are designed to address contamination issues. These products simplify the process and significantly reduce the chances of contamination.
lyphilized exosomes from human MSC (ADSC)
Amount of particles: 4x 10E11 exosomes/mL
Main population particle size: about 95nm
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-08-01
Dokumente:
ProduktbeschreibungQuelle: NCBI PubMed