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AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
Assay: >90% (HPLC); Identity determined by: NMR; Appearance: brown solid; Soluble in: DMSO, DMF; C12H11NO4; MW = 233.22 g/mol. Fluorescence: λex 350 nm; λem 443 nm in methanol.
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Klassifizierungen / ClassificationCAS-Nr: 106562-32-7