Prestained Protein Marker - 10-245 kDa
Sipariş numarası: S5331.0500
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The Prestained Protein Marker - 10-245 kDa is a three-color protein standard with 12 pre-stained (covalently coupled to a chromophore) purified proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kDa and one red one at 75 kDa respectively, when separated on SDS-PAGE (Tris-glycine buffer).
The protein bands represent 10 kDa, 15 kDa, 20 kDa, 25 kDa (green), 35 kDa, 45 kDa, 60 kDa, 75 kDa (red), 100 kDa, 140 kDa, 180 kDa and 245 kDa. The Prestained Protein Marker 10-245 kDa is delivered in convenient 2 x 250µL aliquots. With a recommended application load of 3-5μL per lane, the marker lasts for up to 100 gels.
- 12 protein bands from 10 to 245 kDa - 12 sharp bands with 3 colors - Reference bands at 25 kDa and 75 kDa - Ready-to-use due to integrated loading buffer (no denaturation, no heating necessary) - 500 µL sufficient for 100 mini-gels or 50 large gels
Technische Daten:
Specifications:
Purity: >95% (SDS PAGE)
Ready-to-use protein marker
Number of bands: 12
Protein sizes: 10 kDa, 15 kDa, 20 kDa, 25 kDa, 35 kDa, 45 kDa, 60 kDa, 75 kDa, 100 kDa, 140 kDa, 180 kDa, 245 kDa.
All protein bands are prestained. All blue with the exception of 25 kDa (green) and 75 kDa (red)
Storage buffer: 20mM Tris-phosphate pH7.5 at 25°C, 2% SDS, 1mM β-mercaptoethanol, 3.6M urea and 15% glycerol.
We recommend to load 3-5µL per lane.
Applikation:
- Visualization of protein migration during SDS-polyacrylamide gel electrophoresis - Verification of protein transfer onto membranes after Western blotting - Approximate sizing of proteins on SDS-polyacrylamide gels and Western blotsQuelle
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-04-03Dokumente - Protokolle - Downloads
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