Glycogen solution DNase-free - 20mg/mL

Glycogen is a branched carbohydrate that can be used as a co-precipitant in nucleic acid isolation.

Features of Glyogen:

• Ideal for RT-PCR
• Increases Pellet-Mass
• Quantitative recovery in case of low/very low nucleic acid amounts (ng/mL).
• Prevents pellet loss in nuclease protection assays.
• 260/280nm measurements are unaffected by Glycogen.

Co-precipitants are inert substances (Glycogen or linear Acrylamide) to aid in the recovery of nucleic acids after alcohol precipitation. They are almost indispensable for the quantitative recovery of small quantities of nucleic acids from diluted solutions. Often, the use of such molecules is not desirable for any reason other than the visualization of the pelleted after centrifugation.

When used at a final concentration of 50 to 150µg/mL, glycogen will co-precipitate with nucleic acids in the presence of 0.5M ammonium acetate and isopropanol or ethanol.