10X Phosphate buffered saline powder (pH7.4) - 5 bags



Sipariş numarası: D2016.1005

Shipping: Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

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107,12 € *

pack size:

Please select the favoured pack size.

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and... daha fazla
Ürün bilgileri "10X Phosphate buffered saline powder (pH7.4) - 5 bags"

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns.

This PBS buffer is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches.

There are seventeen standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH, 7.2 and 7.4 and from PBS with or without potassium. PBS without potassium is available in two different molar strengths, 0.01M and 0.02M.

If our internet page doesn't shows the buffer you need, just ask us: info@genaxxon.com.

How to prepare buffer solution:

Empty one pouch of NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL to 300mL of deionized water for a 1000mL pouch and stir the solution for a few minutes. Adjust the volume up to 1000mL, stir and the buffer solution is ready to use.

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Specifications: Purity of used chemicals: min. 99.0%. Content of 1 pouch dissolved in... daha fazla
 

Technische Daten:

Specifications:
Purity of used chemicals: min. 99.0%.

Content of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.10M phosphate buffer, 0.027M potassium chloride, 1.40M sodium chloride, pH7.4 at 25°C (pH of 1-time concentration).

Applikation:

- Wash buffer in immunolabelling techniques, such as ELISA and Western blotting - Blocking buffer for plate based assays - Protein-plate coating

Quelle

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-04-90
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