4,5-Diaminofluorescein - DAF-2

4,5-Diaminofluorescein - DAF-2

Sipariş numarası: S5439.0001

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CAS-Nr: 205391-01-1

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DAF-2 (4,5-Diaminofluorescein) shows higher photostability than the classical fluorescein... daha fazla
Ürün bilgileri "4,5-Diaminofluorescein - DAF-2"

DAF-2 (4,5-Diaminofluorescein) shows higher photostability than the classical fluorescein derivative DAF. Applications: DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxideproducing neurons in brain slices, in mitochondria and in living plant cells.

In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T (Cat-No. S5442 >)).

References: (1) M. Kirsch et al.; J. Pineal Res. 40(1), 10 (2006), (2) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003), (3) N. Nagata et al.; J. Biochem. 125(4), 658 (1999), (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.; Anal. Chem. 70, 2446 (1998)

İlgili bağlantılar "4,5-Diaminofluorescein - DAF-2"
Specifications: Assay: min. 95% (HPLC) Appearance: Light yellow powder Identity: determined by... daha fazla

Technische Daten:

Assay: min. 95% (HPLC)
Appearance: Light yellow powder
Identity: determined by NMR
MW = 362.34 g/mol
Fluorescence: λex 505 nm; λem 518 nm in 0.1M Tris pH8.0
Solubility: soluble in DMSO



Sicherheits Hinweise / Safety

H-Sätze: H302
R-Sätze: R22
Gefahrstoffkürzel: Xn

Klassifizierungen / Classification

CAS-Nr: 205391-01-1
eclass-Nr: 30-19-60-90
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Dokumente - Protokolle - Downloads

Hier finden Sie Informationen und weiterführende Literatur zu 4,5-Diaminofluorescein - DAF-2. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.

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Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
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Quelle/Source: NCBI PubMed >

Conformational and functional characterization of artificially conjugated non-canonical ubiquitin dimers
Tobias Schneider, Andrej Berg, Zeynel Ulusoy, Martin Gamerdinger, Christine Peter, Michael Kovermann
Sci Rep. 2019; 9: 19991. Published online 2019 Dec 27. doi: 10.1038/s41598-019-56458-z
PMCID: PMC6934565

C-Terminal Tyrosine Residue Modifications Modulate the Protective Phosphorylation of Serine 129 of α-Synuclein in a Yeast Model of Parkinson's Disease
Alexandra Kleinknecht, Blagovesta Popova, Diana F. Lázaro, Raquel Pinho, Oliver Valerius, Tiago F. Outeiro, Gerhard H. Braus
PLoS Genet. 2016 Jun; 12(6): e1006098. Published online 2016 Jun 24. doi: 10.1371/journal.pgen.1006098
PMCID: PMC4920419