5-TAMRA-SE / 5-Carboxytetramethylrhodamine N-succinimidyl ester

Amine-reactive carboxytetramethylrhodamine is one of the most commonly used red fluorescent dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm). This single isomer amine-reactive red fluorescent derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.