DIB-Cl / 4-(4,5-Diphenyl-imidazol-2-yl)benzoyl-chloride

4-(4,5diphenyl-1H-imidazol-2-yl)-benzoyl chloride (DIB-Cl) as reagent for amines was used successfully to derivatize and fluorescence detection enantiomers of methamphetamin and phydroxymethamphetamin, in urine samples. DIBCl showed superior fluorescence intensity (FI) to other commercially available reagents, excellent stability in solution and high reactivity with primary and secondary amines under mild conditions. DIB-Cl as a label was used on fluorescent derivatization some hydroxyl group containing endocrine disruptors, including bisphenol A (BPA), bisphenol B (BPB), bisphenol E (BPE), bisphenol F (BPF) and 4-nonylphenol (4NP).