Sipariş numarası: CP-0219-Z025Shipping: Shipment: not cooled. Store at +15°C to +30°C.
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Die CentriPure CP-0219 columns are specially designed for purification and desalting of oligonucleotides longer than 20 base pairs and from proteins greater than 25 kDa with minimal dilution.
The gel bed has a volume of 0.5mL. Samples with a volume up to 100µL can be processed. Optimal purification and recovery is obtained with a sample volume of 50µL.
As the columns are wedded shortly before use the shelf life of the columns is several years at RT.
More than 99% of impurities as salts, dNTPs or Dye terminators can be removed by our CentriPure columns. As a result you will get consitent and reliable signals from base 1 to 700.
- Purification of DNA sequencing reactions.
- Removal of free and labelled dNTP’s from DNA/RNA polymerisation reactions (e.g. RT-PCR, PCR, nick translation).
- Purification/desalting of proteins. Removal of traces of phenol or exchange of buffer salts, as in multiple restriction digestions. These columns are far superior - in ease of use, speed, and non-toxicity - to such common techniques as phenol/chloroform extractions and ethanol precipitations.
Gel Matrix: Zetadex-50SF
Gel bed volume: 0.5mL
Buffer conductifity: Less than 300µS/cm
Sample volume: 20µL to 100µL
Optimal sample size 20µL to 50µL
DNase activity: Not detected;
Applikation:- Purification of DNA sequencing reactions. - Removal of free and labelled dNTP’s from DNA/RNA polymerisation reactions (e.g. RT-PCR, PCR, nick translation). - Purification/desalting of proteins. Removal of traces of phenol or exchange of buffer salts, as
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
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