CentriPure Mini Dye Terminator columns

Die CentriPure CP-0219 columns are specially designed for purification and desalting of oligonucleotides longer than 20 base pairs and from proteins greater than 25 kDa with minimal dilution.

The gel bed has a volume of 0.5mL. Samples with a volume up to 100µL can be processed. Optimal purification and recovery is obtained with a sample volume of 50µL.

Because the columns are rehydrated shortly before use, their shelf life (non-hydrated) at +15°C to +30°C is several years.

More than 99% of impurities as salts, dNTPs or Dye terminators can be removed by our CentriPure columns. As a result you will get consitent and reliable signals from base 1 to 700.

Applications:

- Purification of DNA sequencing reactions.
- Removal of free and labelled dNTP’s from DNA/RNA polymerisation reactions (e.g. RT-PCR, PCR, nick translation).
- Purification/desalting of proteins. Removal of traces of phenol or exchange of buffer salts, as in multiple restriction digestions. These columns are far superior - in ease of use, speed, and non-toxicity - to such common techniques as phenol/chloroform extractions and ethanol precipitations.