rec. Human Interleukin-22 (rHuIL22)

IL-22 is a member of the IL-10 family of regulatory cytokines. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-beta/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family.

Recombinant Human IL-22 produced in E.coli is a non-glycosylated homodimeric polypeptide of 2 x 147 amino acids and a molecular weight of 36.6 kDa.

Amino acid sequence
MAPISSHCRL DKFNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNCAI

Biological activity
The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3ng/mL, corresponding to a specific activity of >3.3 x 10E6 IU/mg.