rec. Human Stem Cell Factor (rHuSCF)

Recombinant Human SCF produced in E.Coli is a single, non-glycosylated polypeptide chain of 164 amino acids and a molecular mass of 18,409 Dalton. Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.

Synonyms:
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Biological Activity:
The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is <2ng/mL, corresponding to a Specific Activity of 5 x 10E5 IU/mg.