IPTG, dioxane free, Molbio Grade
Sipariş numarası: M3198.0010
Shipping: Shipment: not cooled. Stored at +2°C to +8°C. For laboratory usage only!CAS-Nr: 367-93-1
Bugün gönderilmek için hazır,
Teslim süresi yaklaşık olarak 1-3 iş günü
Fiyatlar artı KDV artı nakliye maliyetleri
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal > (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal > (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Mode of operation:
While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.
Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.
Technische Daten:
Specifications:
Purity: min. 99% (HPLC)
Appearance: white powder
RNases, DNases: not detected
MP = 110-112°C
Specific rotation (a20°/D, c=1 water): -20.0° - -34.0°
Water: max. 0.5%
Dioxane: 1.4-Dioxane not detected
Inhibitor free: tested active in Lac-gene expression
C9H18O5S
MW = 238.3 g/mol
Applikation:
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.Quelle
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
EC-Nr: 206-703-0CAS-Nr: 367-93-1
eclass-Nr: 32-16-04-90
Dokumente - Protokolle - Downloads
Hier finden Sie Informationen und weiterführende Literatur zu IPTG, dioxane free, Molbio Grade. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.