X-Gal / 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside

X-Gal / 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside


Order number: M3204.0250

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CAS-Nr: 7240-90-6

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X-Gal (5-Brom-4-chlor-3-indolyl-β-D-galactopyranosid) is a chromogenic substrate for... more
Product information "X-Gal / 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside"
X-Gal (5-Brom-4-chlor-3-indolyl-β-D-galactopyranosid) is a chromogenic substrate for β-galactosidase. It produces a blue color that can easily be detected visually. X-Gal is used in the identification of lacZ + bacteria, especially for the detection of ß-galactosidase, which is expressed by the recombinant vectors. X-Gal is often used in conjunction with IPTG (for the induction of the lac promoter (lac operon)), for the screening of blue colonies. It is also used for the screening of ß-galactosidase reporter gene activity in transfections of eukaryotic cells. X-Gal is a substrate of ß-galactosidase, which releases by cleavage of the substrate 5-bromo-4-chloro-3-indoxyl (X). The enzymatically cleaved indoxyl is oxidized to the insoluble indigo. As a stock solution, for example, a 20mg/mL X-Gal solution in dimethylformamide can be prepared. For the use in LB / Amp - plates this stock solution is to be diluted 1 : 1000 (final concentration 20μg/mL) or 1: 500 (final concentration 40μg/mL). The heated medium should thereby be cooled to about 50°C. The amount of X-Gal may be reduced if an X-Gal solution in DMF is sprayed to the agar plates (already with bacterial colonies!). X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
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Assay: min. 98% (HPLC); Appearance: white to off-white powder; C14H15BrClNO6; MW = 408.6 g/mol;... more
 

Technical Data:

Assay: min. 98% (HPLC); Appearance: white to off-white powder; C14H15BrClNO6; MW = 408.6 g/mol; MP: 223°C; a20°C/D (1%, 50% DMF): -62° +/- 2°; water (K.F.): max: 1%.

application:

- Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies. - Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells. - Detection of beta-galactosidase activity in immunological and histochemical procedures.

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

EC-Nr: 230-640-8
CAS-Nr: 7240-90-6
eclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

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