Lambda-DNA (undigested)
Sipariş numarası: M3073.0500
Shipping: Shipment: on wet ice. Store at -20°C. For laboratory usage only! Bugün gönderilmek için hazır,
Teslim süresi yaklaşık olarak 1-3 iş günü
Fiyatlar artı KDV artı nakliye maliyetleri
Restriction enzyme-digested lambda DNA (48,502bp) generates molecular weight size markers routinely used in gel analysis of nucleic acids. The complete nucleotide sequence has been determined. Lit.: Sanger, F. et al. (1982) J. Mol. Biol. 162, 729.
Details about the DNA sequence can be found in the data sheet attachement.
Description/Preparation:
The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 10E6 daltons and is 48,502 base pairs in length.
The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10mM Tris-HCl (pH7.4) and 1mM EDTA.
The product is methylated.
- GenLadders for DNA sizing cover a broad range of DNA molecular weights - Sharp bands, brigth reference bands - Ready-to-use ladders are stable for 6 months at room temperature
Technische Daten:
Specifications:
Concentration: 0.2 to 0.5mg/mL
Storage buffer: 10mM Tris/HCl (pH7,4), 10mM NaCl, 1mM EDTA.
Applikation:
Can be used for preparation of molecular weight standard for DNA electrophoresis.Quelle
E.coliSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Hier finden Sie Informationen und weiterführende Literatur zu Lambda-DNA (undigested). Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.
Dokumente - Protokolle - Downloads
Is the Genaxxon bioscience Lambda DNA methylated?
Lambda DNA from Genaxxon bioscience is partially methylated. However, we have not determined the actual degree of methylation. In general, not all DNA isolated from E.coli is methylated to the same degree. While pBR322 DNA is fully modified, only about 50% of the possible methylation site of lambda DNA are methylated, presumably because the methylase does not have an opportunity to fully methylate the DNA before it is packaged into the phage head.
