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3'-Deoxythymidine-5'-O-triphosphate (2',3'-Dideoxythymidine-5'-O-triphosphate) (dTTP/ddTTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. Note: Since “thymidine“ already describes a 2'- deoxy nucleosides, the term “2'-deoxythymidine“ is redundant.
3'-Deoxythymidine-5'-O-triphosphate (dTTP/ddTTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. dTTP/ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases.
Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977).
dTTP/ddTTP is an analogue of thymidine triphosphate (TTP) where both ribose hydroxy groups have been removed and replaced by hydrogen.
Amount and packaging:
The product is delivered as 500µL of a10mM solution, pH7.6 (500µL correspond to 5µmol (appr. 2.3mg)).
Typical purity is better than 95% (HPLC / UV / 267 nm) at time of quality control and packing. However, actual purity depends on storage and transport conditions. The product is not sterile and has not been tested for endotoxins.
Purity: min. 95% (HPLC)
10mM solution, pH7.6 (non-sterile, not tested for endotoxins)
λmax = 267 nm; ε = 9600 λ x mmol-1 x cm-1
MW = 466.2 g/mol (free acid)
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 128524-26-5
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