ready-to-use IPTG solution - 10 x 1 mL

Ready-to-use 100mM IPTG solution (Isopropyl-ß-D-thiogalactopyranoside) for molecular biology research.

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.

Shipped as 10 x 1mL solution.