AQ97 High Fidelity MasterMix 2X

AQ97 High Fidelity DNA Polymerase MasterMix 2X is a ready-to-use 2x PCR mix composed of AQ97 High Fidelity DNA Polymerase and an optimised buffer system including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets. The DNA binding domain of this polymerase, ensures excellent high fidelity, long range capacity and also fast amplification results. AQ97 High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.

For longer targets than 11 kb we recommend to use our AQ97 High Fidelity DNA Polymerase with a capability of amplifying DNA targets up to 18 kb.. For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.

AQ97 High Fidelity DNA Polymerase is a novel proofreading DNA polymerase. AQ97 High Fidelity DNA Polymerase is a fused protein complex of DNA polymerase with a processivity-enhancing DNA binding domain. Alongside very fast and robust amplification of complex and long targets, AQ97 High Fidelity DNA Polymerase displays a high fidelity ensuring accurate amplification. The enzyme is well suited for PCR experiments that require amplification with very low error rates, such as cloning/sub-cloning, NGS applications, SNP analysis and mutagenesis.

The AQ97 master mix performs robust amplification of a vast range of difficult targets, including those up to 11 kb and with high to low GC content. The fidelity of the polymerase is more than 60x Taq DNA Polymerase. AQ97 High Fidelity DNA Polymerase 2X Master Mix is recommended for applications, which require extremely high fidelity such as cloning/Sub-cloning, NGS applications, SNP analysis and mutagenesis.

Features:
• High Fidelity: > 60x Taq fidelity
• High elongation rate: 10 sec/kb
• Long range amplification: 11 kb for human gDNA
• 3’ to 5’ proofreading exonuclease activity

Comparison figures of fidelity values for AQ97 DNA Polymerase, AccuPol DNA Polymerase, two well-recognized high fidelity DNA polymerases P and Q and Taq DNA Polymerase were determined through NGS-based analysis of nucleotide misincorporation during PCR. Initially, PCR amplification was performed on a ~ 200 bp synthetic DNA target, generating PCR products for each of the tested polymerases (using recommended setup conditions). For details see file ??? (document section)