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AQ97 High Fidelity DNA Polymerase is a proofreading enzyme for robust amplification of DNA targets with low to high GC content and long DNA targets of 18 kb. The DNA binding domain of this polymerase, ensures excellent high fidelity, long range capacity and also fast amplification results. AQ97 High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.
We recommend to use our AQ97 High Fidelity DNA Polymerase with a capability of amplifying DNA targets up to 18 kb. For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.
AQ97 High Fidelity DNA Polymerase is a novel proofreading DNA polymerase. AQ97 High Fidelity DNA Polymerase is a fused protein complex of DNA polymerase with a processivity-enhancing DNA binding domain. Alongside very fast and robust amplification of complex and long targets, AQ97 High Fidelity DNA Polymerase displays a high fidelity ensuring accurate amplification.
The enzyme is well suited for PCR experiments that require amplification with very low error rates, such as cloning/sub-cloning, NGS applications, SNP analysis and mutagenesis.
• High Fidelity: > 60x Taq fidelity
• High elongation rate: 10 sec/kb (up to 6000bp per minute)
• Long range amplification: 18 kb for human gDNA
• 5'-3' polymerase acitivity and 3'-5' exonuclease activity.
• generates blund ends.
Comparison figures of fidelity values for AQ97 DNA Polymerase, AccuPol DNA Polymerase, two well-recognized high fidelity DNA polymerases P and Q and Taq DNA Polymerase were determined through NGS-based analysis of nucleotide misincorporation during PCR. Initially, PCR amplification was performed on a ~ 200 bp synthetic DNA target, generating PCR products for each of the tested polymerases (using recommended setup conditions). For details see file ??? (document section)
High Fidelity: > 60x Taq DNA Polymerase • High elongation rate: 10 sec/kb • 3’ → 5’ proofreading exonuclease activity. • Good coverage on difficult DNA templates with low to high GC content • Long range capability: 11 kb for gDNA
• High Fidelity: >60x Taq DNA Polymerase
• High elongation rate: 10 sec/kb
• 3’ → 5’ proofreading exonuclease activity.
• Good coverage on difficult DNA templates with low to high GC content
• Long range capability: 18 kb for gDNA
• delivered as 2 units/µL enyzme solution
Applikation:PCR for cloning, subcloning, mutagenesis, NGS applications, SNP analysis and mutagenesis
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-05-02
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