B-Enhancer solution

B-Enhancer solution

Sipariş numarası: M3041.0500

Shipping: Shipment: not cooled. Store at -20°C. For laboratory usage only!

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The better performance of the Genaxxon bioscience 5X B-Enhancer Solution compared to standard... daha fazla
Ürün bilgileri "B-Enhancer solution"

The better performance of the Genaxxon bioscience 5X B-Enhancer Solution compared to standard enhancers such as form amide, DMSO, TMCA or BSA especially when used with GC rich regions or templates with a high degree of secondary structures depends on its ability to lower the DNA melting temperature and its enhancing effect on the DNA polymerase.
In detail, Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level. At the same time, Betaine has a sequence independent destabilizing effect on all DNA. Summarized, the Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered. Furthermore, Betaine aids the processivity of thermostable polymerases and reduces "pauses" in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.
As Betaine has a decreasing effect on the melting temperature of DNA and primers the denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5°C compared to the annealing temperature already used. The optimal annealing temperature should be determined individually for each reaction.

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Specifications: contains 5M betaine. daha fazla

Technische Daten:

contains 5M betaine.

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
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Hier finden Sie Informationen und weiterführende Literatur zu B-Enhancer solution. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.