DMEM for SILAC(TM) w/o Glutamine, w/o Arginine, w/o Lysin, with 4.5g/L Glucose

Dulbecco's Modified Eagle Medium (DMEM), with 4.5g/L glucose, no glutamine, no lysine, no arginine is a basal cell culture medium for use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. Special preparation. Minimum order size: 20 x 500mL.

DMEM for SILAC™ has been specifically formulated for use with stable isotope labelling with amino acids in cell culture (SILAC) for the analysis of protein expression by mass spectrometry (MS).
SILAC™ is a powerful method for identifying and quantifying even differential changes in complex protein samples. The approach involves in-vivo integration of stable 13C- or 15N-labelled amino acids into proteins, followed by mass spectrometry (MS) for rapid and comprehensive protein identification, characterisation and quantification.

DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells.

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com.

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