Protease Inhibitor Mix V for Tissue extracts

Mixture of 6 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with extracts from mammalian tissue, but can also be used with other extracts.

This ready-to-use mix is free of EDTA and contains AEBSF >, Aprotinin >, E-64 >, Leupeptin >, Bestatine >, and Pepstatin A >. The kit is supplied with an extra vial of 1mL ultrapure DMSO.

The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL extract.

Activity spectrum
AEBSF is an inhibitor against Serine proteases. Aprotinin is an inhibitor against Serine proteases. E-64 is an inhibitor against Cysteine proteases. Leupeptin is an inhibitor against Cystein proteases and trypsin-like Serine proteases. Peptstatin A is an inhibitor against Aspartic proteases. Bestatin is an inhibitor against Aminopeptidase B and Leucine-Aminopeptidases.

More ready-to-use protease inhibitor mixes can be found here:

- Protease-Inhibitor-Mix I > (general applications, contains no EDTA)
- Protease-Inhibitor-Mix II > (general applications, contains EDTA)
- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)
- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)
- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA)

You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.