IdentityTaq 2X Master Mix for food of animal origin
Sipariş numarası: M3120.0100
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Direct PCR amplification of meat-or milk-specific DNA sequences from prepared food containing beef, pork, lamb, poultry or turkey, without the need for prior DNA isolation or DNA purification!
The Genaxxon bioscience IdentityTaq 2X Mastermix contains an optimized polymerase that is highly resistant to PCR inhibitors. Fatty samples, such as e.g. Salami, cheese or sausage can be analyzed directly and without prior processing. Other problematic samples, such as wild animal hair or saliva, can thus be easily examined. This master mix is delivered without lysis buffer. If you need the IdentityTaq PCR 2X master mix with lysis buffer, please refer to the M3089 >.
Advantages:
- The IdentityTaq polymerase that is used in our IdentityTaq master mix has been specifically optimized to tolerate a variety of PCR inhibitors that otherwise prevent direct PCR from cell extracts.
- The IdentityTaq 2X master mix ensures reproducible results, and significantly reduces set-up times and the risk of pipetting errors.
NOTE: Due to the use of the specifically engineered Hotstart DNA polymerase and the specific buffer used for the IdentityTaq 2X PCR master mix the use of protocols typically already established in the laboratory is highly likely to result in reaction failure as buffer compositions are different from that used before.
Additional PCR master mixes can be found here: qPCR/realtime master mixes > and Standard PCR master mixes >.
For more information on our product with a direct PCR test for food of animal origin, see our YouTube video:
Watch the video
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Datenblätter
- No need for time-consuming and expensive DNA purification steps - Very little sample material required - Special DNA Polymerase provides high yields of specific product.
Technische Daten:
Specifications:
2-time master mix for direct PCR of meat-specific DNA sequences from prepared food (Hamburger, Hotdog, Döner Kebap, etc.).
1.25mL is sufficient for 100 x 25µL PCRs.
1.25mL of 2X master mix
Applikation:
• Laboratory analyses of livestock food; pet food; meat (cooked, boiled, fresh, stored at +2°C to +8°C or frozen) • Direct gene detection and quantification • Screening / High-throughput PCRsQuelle
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Hier finden Sie Informationen und weiterführende Literatur zu IdentityTaq 2X Master Mix for food of animal origin. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.
Dokumente - Protokolle - Downloads
Due to the use of the specifically engineered Hotstart DNA polymerase and the specific buffer used for the IdentityTaq 2X PCR master mix the use of protocols typically already established in the laboratory is highly likely to result in reaction failure as buffer compositions are different from that used before.
The proprietary lysis buffer is different from that routinely used with Proteinase K, for tissue digestion and subsequent PCR, and the annealing temperatures required for subsequent PCR are also likely to be different. We recommend to make a gradient PCR first!
IdentityTaq Mastermix with Animal Hair:
If there is little source material and the PCR results are weak, the following tips may be helpful:
- Reduce lysis buffer: 100μL instead of 200μL
- increase the number of cycles (up to 45)
- Use more lysate for the batch: 4μL instead of 2μL
