M-MuLV Reverse Transcriptase RNase H negative
Sipariş numarası: M3042.1010
Shipping: Shipment: on wet ice. Store at -20°C. For laboratory usage only! Bugün gönderilmek için hazır,
Teslim süresi yaklaşık olarak 1-3 iş günü
Fiyatlar artı KDV artı nakliye maliyetleri
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. M-MuLV RT will also extend primers hybridized to single-stranded DNA!
The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
Genaxxon offers the enzyme HotScriptase (M3056) > and the HotScriptase master mixes (M3062 >; M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!
Money saving: high!
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
Free of RNase activity resulting in an increase of full-length cDNA products. M-MuLV is used in cDNA synthesis with long templates.
Technische Daten:
Specifications:
Activity: 200 - 400 CEL units/µL.
Purity: Free of RNase activity resulting in an increase of full-length cDNA products.
Purity: >98% protein homogeneity in 10% SDS-PAGE electrophoresis.
MW = 69 kDa.
Enzyme is shipped together with complete buffer.
Applikation:
reverse transcription cDNA synthesisQuelle
E.coliSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Hier finden Sie Informationen und weiterführende Literatur zu M-MuLV Reverse Transcriptase RNase H negative. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.
