Total RNA Purification Mini Spin Kit

Genaxxon´s Total RNA Purification Mini Spin kit is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) and 10E4-10E7 cultured cells! Comparable with Qiagen RNeasy Mini Kit (74104).

Genaxxon´s Total RNA Purification Mini Spin kit is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) and 10E4-10E7 cultured cells. The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Mini Spin kit utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed. Any RNases are inactivated. The three-step washing stage effectively removes impurities and enzyme inhibitors.

The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.

Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.

You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.

With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!

Please check also our DNA purification kits >.