5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester / 5(6)-Carboxy-DCF DA SE

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

References: (1) S.J. Bark et al.; J. Am. Chem. Soc. 122, 3567 (2000), (2) P. Hoogerhout et al.; J. Pept. Res. 54, 436 (1999), (3) Y. Wang et al.; Biochemistry 35, 12338 (1996).