One-Step RT-qPCR 2X ProbeMastermix

The One-Step RT-qPCR 2X ProbeMastermix without ROX® was developed for quantitative real-time analyzes of RNA templates with double-labelled fluorescence probes. The ready-to-use mixture is based on an optimized genetically modified reverse transcriptase with improved thermal stability, which offers increased specificity, a high cDNA yield and improved efficiency for highly structured and long cDNA fragments.

The 2X Mastermix contains all the reagents required for the RT-qPCR (except for the template, primer and probes) to ensure quick and easy handling at a minimum of pipetting steps. The high-quality enzymes and the optimized reaction buffer with high-purity dNTPs guarantee superior real-time PCR results.

RT-qPCR is used to amplify double-stranded DNA from single-stranded RNA templates to enable rapid real-time quantification of RNA targets. In the reverse transcription step, the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA). The cDNA molecules are amplified by the HotStart DNA polymerase. The HotStart polymerase activity is blocked at ambient temperature and will be activated at the beginning of the initial denaturation. This thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during the PCR setup.

One-Step RT-qPCR offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination.

Genaxxon offers the enzyme HotScriptase (M3056) > and the HotScriptase master mixes (M3062 >, M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!
Money saving: high!

> frequently asked questions