Genaxx1Step RT-qPCR COVID-19 CDC Probe Assay Kit

The Genaxx1Step RT-qPCR SARS-CoV-2 CDC Probe Assay Kit is a real-time RT-PCR-based detection system for the targets N1 and N2 of the nucleocapsid from the SARS-CoV-2-Corona virus (2019-nCoV), as well as for the control target RNASeP. The kit has been optimized for qualitative in vitro detection according to the US CDC (Centers for Disease Control and Prevention).

The Genaxx1Step RT-qPCR SARS-CoV-2 CDC Probe Assay Kit consists of the innovative HotScriptase Covid-19 master mix with its very thermostable polymerase. Primers and probes according to CDC-authorized sequences for the N1, N2 assays and for RNASeP can be purchased, for example, from IDT (Integrated DNA Technologies) or Eurofins.

The test is not affected by following main variants (will detect these variants): BA 2.75, XBB 1.5.

Genaxx1Step Covid-19 master mix enables the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without an isothermal reverse transcription step. The light blue color improves the visibility of the mixture during the pipetting steps, especially if white or transparent PCR plates or PCR tubes are used. (find out more about our realtime master mixes >)

• faster: One-Step - NO time consuming isothermal intermediate step necessary.
• easier: reverse transcriptase and DNA polymerase in one single enzyme; reverse transcription and DNA amplification in parallel during the PCR elongation step. You need only a PCR protocol.
• saver: much reduced contamination risk, due to reduced pipetting steps.
• extreme thermostablility: secondary structures will be a minor problem due to higher temperatures of PCR compared to reverse transcription reactions.
• cheaper: parallel reverse transcription and amplification by HotScriptase RT saves time and costs.

The Genaxxon bioscience HotScriptase can be ordered as HotScriptase DNA polymerase > with 5 units/µL or as a convenient 2-time RT master mix. The 2-time RT master mix contains all necessary ingredients for RT-PCR without specific primers, probes or RNA/DNA. Additionally Genaxxon offers HotScriptase RT Cell master mix >, a 2-time master mix designed for direct RT-PCR from whole cells! With our HotScriptase RT Cell master mix you can skip time consuming and expensive RNA isolation and purification starting the RT-PCR direct from cells (mammals).

The Genaxxon HotScriptase DNA polymerase > for reverse transcription enables a "One-Step” RT-PCR directly from cell suspensions or mammals cells without an isothermal reverse transcription intermediate step. Just give cells or cell suspension (100 up to <10,000 cells) directly to the PCR reaction mixture, mix, place in a thermal cycler and start PCR program.

As there is no need for expensive and time consuming cell lysis, isothermal transcription or RNA purification, the Genaxxon HotScriptase Cell RT master mix > is ideally suited for High-throughput applications as RNAi knockdown, gene expression studies or realtime PCR (qPCR). The ready-to-use 2-time master mix ensures reproducible RT-PCR results in addition to very much reduced setup times and the reduced risk of contamination due to pipetting errors.

HotScriptase RT polymerase is a biotechnical engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution. The HotScriptase 2-time master mix is a ready-to-use mixture of the propietory HotScriptase, buffer, dNTPs and special components. The recommended amplicon size should be between 60-400 bp!

This master mix contains no ROX nor green dye!

> find out about our realtime master mixes
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> frequently asked questions (FAQs)