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TEMPase Hot Start DNA Polymerase

Units

Hersteller Ampliqon A/S



Artikel-Nr.: A221102

shipped on blue-ice, store at -20°C

 

Sofort versandfertig, Lieferzeit ca. 1-2 Werktage

90,00 € *
 
 
 
 
 

Produktinformationen "TEMPase Hot Start DNA Polymerase"

About TEMPase Hot Start DNA Polymerase

TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase allows for detection of low copy number targets and for multiplex PCR in a single reaction tube. TEMPase Hot Start DNA Polymerase is available together with different buffers. This one (A2211xx) is supplied with ammonium sulfate and MgCl2 containing buffer plus separate 25mM MgCl2.

TEMPase Hot Start DNA Polymerase is also available together with ammonium sulfate containing buffer plus combination buffer plus MgCl2 (A2251xx).

Features

    - Reaction set up at room temperature
    - Increased sensitivity
    - Increased specificity
    - Increased product yield

Description

TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended.

Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a heat activation step, releasing the active TEMPase Hot Start DNA Polymerase into the reaction.

Mit unseren hochwertigen dNTPs als Set (M3015.4100 und M3015.0250) oder als Mix (M3016.1010) oder unseren DNA-Markern und unserer günstigen Standardagarose bieten wir Ihnen weitere Produkte für die PCR.

 

 

Applikation:

For multiplex PCR. For Real-time PCR, for PCR of low amount of DNA (very low copy number).
 
 

Technische Daten:

5 units/µL.

Enyzme is completely inactive prior to heating at 95°C for 15 min.!

Applikation:

For multiplex PCR. For Real-time PCR, for PCR of low amount of DNA (very low copy number).

Einheitendefinition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50mM K

Quelle

recombinant

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

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