TEMPase Hot Start DNA-Polymerase

Tempase Hot Start Polymerase


Artikel-Nr.: A223102

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About TEMPase Hot Start DNA Polymerase TEMPase Hot Start DNA Polymerase has been designed to... mehr
Produktinformationen "TEMPase Hot Start DNA-Polymerase"

About TEMPase Hot Start DNA Polymerase

TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase allows for detection of low copy number targets and for multiplex PCR in a single reaction tube. TEMPase Hot Start DNA Polymerase is available together with different buffers. This one (A2231xx) is supplied with 10X combination buffer plus separate 25mM MgCl2.

TEMPase Hot Start DNA Polymerase is also available together with 10X ammonium sulfate buffer and separate 25mM MgCl2 (A2211xx >).

Features

    - Reaction set up at room temperature
    - Increased sensitivity
    - Increased specificity
    - Increased product yield

Description

TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended.

Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a heat activation step, releasing the active TEMPase Hot Start DNA Polymerase into the reaction.

Mit unseren hochwertigen dNTPs als Set (M3015.4100 und M3015.0250) > oder als Mix (M3016.1010) > oder unseren DNA-Markern > und unserer günstigen Standardagarose > bieten wir Ihnen weitere Produkte für die PCR.

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5 units/µL. Enyzme is completely inactive prior to heating at 95°C for 15 min.! mehr
 

Technische Daten:

5 units/µL.

Enyzme is completely inactive prior to heating at 95°C for 15 min.!

Applikation:

for multiplex PCR for Real-time PCR for PCR of low amount of DNA (very low copy number)

Einheitendefinition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Quelle

recombinant

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads mehr

Dokumente - Protokolle - Downloads

Hier finden Sie Informationen und weiterführende Literatur zu TEMPase Hot Start DNA-Polymerase. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.