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CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).
Purity: >98.0% (HPLC); MW = 614.89 g/mol; Appearance: White solid crystals; CMC 4.2 - 6.3 mmol/L; Aggregation No.: 9-10; Conductivity: max. 10μS/cm (25°C, 1% solution); Ca: max. 0.0005%; Cu: max. 0.0005%; Fe: max. 0.0005%; Mg: max. 0.0005%; K: max. 0.005%; Pb: max. 0.005%; Na: 0.005%; C32H58N2O7S.
Sicherheits Hinweise / SafetyH-Sätze: H302, H315, H319
Klassifizierungen / ClassificationCAS-Nr: 75621-03-3
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Here you will find information and further literature on CHAPS buffer grade. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.